cell genomics: human engineering of genes (Introduction)

by David Turell , Wednesday, May 01, 2024, 18:10 (16 days ago) @ David Turell

Using bacterial mechanisms to edit:

https://www.the-scientist.com/mega-crispr-engineering-better-immunotherapies-with-rna-e...

"Many foundational research technologies have transformed cellular therapies, moving treatments from concept to clinic. In the past decade, chimeric antigen receptors (CAR) and genome editing are two standouts that led to breakthrough CAR T cell therapies for leukemia and lymphoma. Scientists engineer these treatments with virus-mediated gene insertion ex vivo, which instructs T cells to express synthetic receptors that detect tumor-specific antigens and guide cancer cell eradication after transplantation. Researchers investigate clustered regularly interspaced short palindromic repeats (CRISPR) editing to improve CAR T cell therapies and expand their applicability to more cancer types. However, CRISPR-associated nuclease 9 (Cas9)-based genome cutting tools face unique safety and efficacy limitations due to the permanent nature of DNA editing.

"To circumvent these limitations, bioengineer Lei (Stanley) Qi and physician immunologist Crystal Mackall at Stanford University developed an RNA editing tool called multiplexed effector guide arrays (MEGA). In a study published in Cell, the team used Cas9’s cousin, Cas13, and a pooled array of guide RNAs to simultaneously edit multiple gene transcripts in primary human T cells without targeting or cutting genomic DNA. This multi-targeting method addresses an unmet need in cell therapy optimization by allowing the researchers to dynamically regulate several pathways per T cell, rather than add or ablate individual genes completely, one at a time. The researchers screened for genes that synergistically affect T cell function and knocked down redundant transcripts that drive T cell exhaustion in culture and in mice.

“'Our years of experience on gene editing at the DNA level makes us realize that this technology, while very powerful, still has some intrinsic challenges, which possibly could only be addressed if we find methods to engineer RNA,” said Qi. Among these difficulties are off-target cuts and accumulation of genomic instability through multiple DNA edits. “RNA is completely different. If we target RNA, we do not touch the DNA all, and RNA editing is reversible,” Qi explained. Another advantage is Cas13’s ability to process several unique guide RNAs from a single array, which allowed the researchers to target multiple RNA transcripts at once in the same cell.

"The research team first put MEGA through its paces in cell culture. To validate the tool, they targeted three previously established inhibitors of T cell function at once, which effectively suppressed T cell exhaustion. The research team also performed a large-scale, combinatorial screen with multiple guide arrays, uncovered new genetic regulators of T cell function, and created a drug-inducible form of MEGA for dose-responsive CAR activation. They then used MEGA to enhance CAR T cell fitness and anti-tumor activity in cancer cell co-cultures and in a mouse model of leukemia by disrupting multiple metabolic pathway components in T cells before transplantation.

“'This is a beautiful paper. It adds another dimension to what we have already been able to do with DNA-targeting CRISPR by taking advantage of this excellent RNA-targeting CRISPR platform,” said Neville Sanjana, a bioengineer at New York University who was not involved in this study. “We need a broad genome engineering toolbox. They're great tools, Cas9 and Cas13, but being able to turn genes on and off, and to do this in multiplexed ways, that's the future that we really want.'”

Comment: Suddenly humans are at a God-the-designer level of actions with the genome. The current focus is on cancer defenses, but the ethical considerations will come into play if editing is at a level which affects personality for example.